Composite

Part:BBa_K5166065:Design

Designed by: ZiWei Xu,siyu zhu   Group: iGEM24_BIT-China   (2024-10-01)


UreE-UreF-UreG-UreD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2448
    Illegal XbaI site found at 47
    Illegal XbaI site found at 927
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2448
    Illegal NheI site found at 1415
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2448
    Illegal BglII site found at 1947
    Illegal BamHI site found at 73
    Illegal XhoI site found at 712
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2448
    Illegal XbaI site found at 47
    Illegal XbaI site found at 927
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2448
    Illegal XbaI site found at 47
    Illegal XbaI site found at 927
    Illegal AgeI site found at 1216
    Illegal AgeI site found at 1379
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

We found the basic ureaseEFGD submit gene from S. pasteurii,the RBS sequences are re-coded based on natural RBS by us to match starting expressing speed for each subpart.

References